Xylopentose production from crop residue employing xylanase enzyme

• Published in: Bioresource technology Vol. 370; p. 128572
• Main Authors: Kaur, Pritam, Khatri, Madhu, Singh, Gursharan, Selvaraj, Manickam, Assiri, Mohammed A., Lalthazuala Rokhum, Samuel, Kumar Arya, Shailendra, Jones, Sumathi, Greff, Babett, Woong Chang, Soon, Ravindran, Balasubramani, Awasthi, Mukesh Kumar
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.2023
• Subjects: Acetic Acid; Biomass; Endo-1,4-beta Xylanases; Glucuronates; Hydrolysis; Lignocellulosic biomass; Oligosaccharides; Secondary crop residue; Sugars; Xylanase; Xylooligosaccharides; Xylanase; Secondary crop residue; Lignocellulosic biomass; Xylooligosaccharides
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2022.128572

Pictorial representation of the methodology carried out for the Green Coconut agro-waste sample. A similar process was carried out for the other four agro-waste samples. [Display omitted] •Two method HP-AC and SH-SH were used for pretreatment of CG and VC.•Secondary crop residue GC was best outcomes were observed for XOS Production.•Xylopentose was obtained in high quantity 96.44% and 93.09% from CG and VC respectively.•Decreasing the co-production of xylose from secondary crop residue. To produce xylo-oligosaccharides (XOS) from the agriculture waste, which included, green coconut and vegetable cocktail. The two pretreatment – hydrogen peroxide-acetic acid (HP-AC) and sodium hypochlorite-sodium hydroxide (SH-SH) – were used for this study. The optimal conditions for the pretreatment were 80 °C, 4.0 % NaClO, and 2 h, followed by 0.08 % NaOH, 55 °C, and 1 h. Further enzymatic hydrolysis of green coconut (GC) and vegetable cocktail (VC) were performed and found in case of GC, the best outcomes were observed. Different types of XOS were obtained from the treated biomass whereas a single type of XOS xylo-pentose was obtained in high quantity (96.44 % and 93.09 % from CG and VC respectively) with the production of other XOS < 2 %. This study presents a reasonably secure and economical method for turning secondary crop residue into XOS and fermentable sugars.