Relationship between bacterial diversity and environmental parameters during composting of different raw materials

• Published in: Bioresource technology Vol. 198; pp. 395 - 402
• Main Authors: Wang, Xueqin, Cui, Hongyang, Shi, Jianhong, Zhao, Xinyu, Zhao, Yue, Wei, Zimin
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.12.2015
• Subjects: Bacteria - genetics; Bacteria - isolation & purification; Bacterial diversity; Composting; Denaturing gradient gel electrophoresis (DGGE); DNA, Bacterial - isolation & purification; Redundancy analysis (RDA); Soil; Soil Microbiology; Composting; Denaturing gradient gel electrophoresis (DGGE); Bacterial diversity; Redundancy analysis (RDA)
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2015.09.041

[Display omitted] •Compare the physical–chemical parameters during different raw materials composting.•Analyze the structure and succession of bacteria during composting.•Confirm the correlation of bacterial diversity with environmental factors.•A strategy to enhance abundance of uncultured bacteria was proposed. The aim of this study was to compare the bacterial structure of seven different composts. The primary environmental factors affecting bacterial species were identified, and a strategy to enhance the abundance of uncultured bacteria through controlling relevant environmental parameters was proposed. The results showed that the physical–chemical parameters of each different pile changed in its own manner during composting, which affected the structure and succession of bacteria in different ways. DGGE profiles showed that there were 10 prominent species during composting. Among them, four species existed in all compost types, two species existed in several piles and four species were detected in a single material. Redundancy analysis results showed that bacterial species compositions were significantly influenced by C/N and moisture (p<0.05). The optimal range of C/N was 14–27. Based on these results, the primary environmental factors affecting a certain species were further identified as a potential control of bacterial diversity.