Structural characterization of a novel polysaccharide from Pleurotus citrinopileatus and its antitumor activity on H22 tumor-bearing mice

• Published in: International journal of biological macromolecules Vol. 168; pp. 251 - 260
• Main Authors: Wang, Qing, Niu, Lu-Lu, Liu, Hui-Ping, Wu, Ya-Ru, Li, Meng-Yu, Jia, Qi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 31.01.2021
• Subjects: Animals; Antineoplastic Agents - pharmacology; Antitumor activity; Apoptosis - drug effects; Arabinose - pharmacology; Cell Cycle - drug effects; Cell Division - drug effects; China; Galactose - pharmacology; Glucuronic Acid - pharmacology; Glycosides - pharmacology; Liver Neoplasms - pathology; Male; Mannose - pharmacology; Mice; Molecular Weight; Monosaccharides - pharmacology; Pleurotus - metabolism; Pleurotus citrinopileatus; Polysaccharide; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Structural characterization; Xylose - pharmacology; China; Pleurotus citrinopileatus; Antitumor activity; Polysaccharide; Structural characterization
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2020.12.053

In this research, a novel polysaccharide (PCP) was extracted from Pleurotus citrinopileatus and purified by Sephadex G-150 gel column, and its antitumor activity was investigated using the model H22 tumor-bearing mice. PCP was found to be composed of arabinose, galactose, glucose, xylose, mannose and glucuronic acid in a proportion of 0.66: 14.59: 10.77: 1: 0.69: 0.23 with average molecular weight of 7.30 × 105 Da. Further analysis suggested that PCP was a pyranose with α-type and β-type glycosidic residues. The antitumor assays in vivo indicated that PCP could effectively suppress H22 solid tumor growth, protect immune organs and improve inflammation and anemia. Besides, Annexin V-FITC/PI double staining and JC-1 staining demonstrated that PCP could induce apoptosis of H22 hepatoma cells. The PI staining assay revealed that PCP induced H22 hepatoma cells apoptosis by arresting cell cycle in S phase. These results suggest that the polysaccharide from Pleurotus citrinopileatus possesses potential value in the treatment of liver cancer.