Solubilization and purification of rat tissue phospholipase D

Rat phospholipase D was successfully solubilized with Triton X-100. Solubilized phospholipase D is clearly separated from phosphatidic acid phosphatase and still dependent on sodium oleate for activity.

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Bibliographic Details
Published inMethods in enzymology Vol. 197; p. 575
Main Authors Kobayashi, M, Kanfer, J N
Format Journal Article
LanguageEnglish
Published United States 1991
Subjects
Animals
Brain - enzymology
Carbon Radioisotopes
Chromatography, Ion Exchange - methods
Chromatography, Thin Layer - methods
Detergents - pharmacology
Indicators and Reagents
Kinetics
Lung - enzymology
Microsomes - enzymology
Phospholipase D - analysis
Phospholipase D - isolation & purification
Phospholipase D - metabolism
Radioisotope Dilution Technique
Rats
Solubility
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Summary:Rat phospholipase D was successfully solubilized with Triton X-100. Solubilized phospholipase D is clearly separated from phosphatidic acid phosphatase and still dependent on sodium oleate for activity.
ISSN:0076-6879
DOI:10.1016/0076-6879(91)97185-2