Genotoxicity of (+)- and (−)-usnic acid in mice

• Published in: Mutation research. Genetic toxicology and environmental mutagenesis Vol. 839; pp. 36 - 39
• Main Authors: Prokopiev, Ilya, Filippova, Galina, Filippov, Eduard, Voronov, Ivan, Sleptsov, Igor, Zhanataev, Aliy
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2019
• Subjects: DNA-comets; Enantiomers; Genotoxcity; Mice; Oxidative stress; Usnic acid; Oxidative stress; Mice; Usnic acid; Enantiomers; DNA-comets; Genotoxcity
• ISSN: 1383-5718; 1879-3592
• DOI: 10.1016/j.mrgentox.2019.01.010

•The genotoxic effect of usnic acid was only observed 1 h after oral administration.•(+)- and (−)-usnic acid doses of 100 and 50 mg/kg resulted in DNA damage in the liver and kidney cells.•The genotoxic effect of usnic acid is associated with oxidative stress.•There were no significant differences in the effects of (+)- and (−)-enantiomers. Usnic acid, which is the most widespread and well-studied secondary lichen compound, has antibacterial and cytotoxic effects. Usnic acid is present in lichens as the (+)- and (-)-enantiomers, which have different biological activities. We used a DNA-comet assay to determine the genotoxic effect of (+)- and (−)-usnic acid in the liver and kidney cells of mice. The genotoxic effect of usnic acid was only observed 1 h after oral administration. Usnic acid doses of 100 and 50 mg/kg resulted in DNA damage in the liver and kidney cells. The genotoxic effect of usnic acid is associated with oxidative stress in cells. There were no significant differences in the effects of (+)- and (−)-enantiomers.