A novel higher polyhydroxybutyrate producer Halomonas halmophila 18H with unique cell factory attributes

• Published in: Bioresource technology Vol. 372; p. 128669
• Main Authors: Aytar Celik, Pinar, Barut, Dilan, Enuh, Blaise Manga, Erdogan Gover, Kubra, Nural Yaman, Belma, Burcin Mutlu, Mehmet, Cabuk, Ahmet
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.03.2023
• Subjects: Cell factory; Fermentation; Halomonas; Halomonas - genetics; Halomonas - metabolism; Halophile; Hydroxybutyrates - metabolism; Polyhydroxyalkanoates - metabolism; Polyhydroxybutyrate; Whole genome analysis; Cell factory; Halophile; Halomonas; Polyhydroxybutyrate; Whole genome analysis
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2023.128669

[Display omitted] •Among 94 Halomonas strains, the highest producer (Halomonas halmophila 18H) yielded 63.72 % PHB.•SEM, FTIR, 1H NMR, XRD, and TGA confirmed PHB structure and its high thermal stability.•The genome is large with several copies and locations PhABC genes.•It had clusters for ectoine, plipastatin, carotenoids, and diutan polysaccharide.•Halomonas sp. 18H is a potential cell factory for many bioproducts. For cost-competitive biosynthesis of polyhydroxybutyrate (PHB), the screening of efficient producers and characterization of their genomic potential is fundamental. In this study, 94 newly isolated halophilic strains from Turkish salterns were screened for their polyhydroxyalkanoates (PHAs) biosynthesis capabilities through fermentation. Halomonas halmophila 18H was found to be the highest PHB producer, yielding 63.72 % of its biomass as PHB. The PHB produced by this strain was physically and chemically characterized using various techniques. Its genome was also sequenced and found to be large (6,713,657 bp) and have a GC content of 59.9 %. Halomonas halmophila 18H was also found to have several copies of PHB biosynthesis genes, as well as 20 % more protein-coding genes and 1075 singletons compared to other high PHB producers. These unique genomic features make it a promising cell factory for the simultaneous production of PHAs and other biotechnologically important secondary metabolites.