Detection of Legionella pneumophila serogroup 1 antigen in respiratory samples using an immunochromatographic membrane test

• Published in: Journal of microbiological methods Vol. 74; no. 2; pp. 121 - 122
• Main Authors: Higa, Futoshi, Koide, Michio, Furugen, Makoto, Akamine, Morikazu, Hibiya, Kenji, Haranaga, Shusaku, Yara, Satomi, Tateyama, Masao, Yamane, Nobuhisa, Fujita, Jiro
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier B.V 01.08.2008; Elsevier Science
• Subjects: Antigens, Bacterial - analysis; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Bronchial washing; Bronchoalveolar Lavage; Chromatography - methods; Diagnosis of community-acquired pneumonia; Fundamental and applied biological sciences. Psychology; Humans; Immunoassay - methods; Immunochromatographic test; Legionella pneumonia; Legionella pneumophila; Legionella pneumophila - classification; Legionella pneumophila - isolation & purification; Legionnaires' Disease - diagnosis; Legionnaires' Disease - microbiology; Microbiology; Sensitivity and Specificity; Sputum - chemistry; Sputum - microbiology; Diagnosis of community-acquired pneumonia; Legionella pneumonia; Bronchial washing; Immunochromatographic test; Legionellaceae; Respiratory disease; Immunoaffinity chromatography; Legionella pneumophila; Method; Antigen; Bacteria; Diagnosis; Detection
• ISSN: 0167-7012; 1872-8359
• DOI: 10.1016/j.mimet.2008.03.009

The immunochromatographic membrane test (ICT) efficacy of Legionella antigen detection (Binax Now Legionella®) was evaluated using respiratory samples, including bronchial washings (44 cases) and sputum (128 cases), from suspected Legionella pneumonia patients. The ICT results using respiratory samples agreed well with isolation of L. pneumophila SG1 and ICT using urines.