Automated isolation and processing of adipose-derived stem and regenerative cells

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 702; p. 87
• Main Authors: Hicok, Kevin C, Hedrick, Marc H
• Format: Journal Article
• Language: English
• Published: United States 01.01.2011
• Subjects: Adipose Tissue - cytology; Automation - methods; Cell Count; Cell Culture Techniques - methods; Cell Separation - methods; Cell Survival; Cells, Cultured; Colony-Forming Units Assay; Culture Media; Humans; Regeneration - physiology; Stem Cells - cytology
• ISSN: 1940-6029
• DOI: 10.1007/978-1-61737-960-4_8

The popularity of nonhematopoietic, adult tissue-derived stem and progenitor cells for use as a cellular research tool, and ultimately as a clinical therapeutic, has increased exponentially over the past decade. Almost all adult-derived stem/progenitor cells (autologous and allogeneic), with one exception, require at least some ex vivo expansion or further manipulation prior to use to satisfy efficacy and safety requirements for preclinical or clinical use. The principal reason is the relatively low frequency of these therapeutically valuable cells within any given adult tissue, except for adipose tissue, which has been shown to have at least two log greater concentrations of these progenitor cells. Therefore, use of autologous adipose-derived cells as both a research tool and cell therapeutic is feasible and has been shown to be both safe and efficacious in preclinical and clinical models of injury and disease. The development and utilization of automated processes and instrumentation such as Cytori Therapeutics' Celution® System to reduce variability and increase quality of the recovered cells is requisite for clinical use and preferred by basic researchers. Here, use of an automated, closed processing platform for isolation and concentration of adipose-derived stem and regenerative cells is described, including a profile of the isolated cells immediately prior to use, and commonly used methods to quantify and qualitatively assess the recovered cells.