Formation of a novel coating material containing lutein and zeaxanthin via a Maillard reaction between bovine serum albumin and fucoidan

• Published in: Food chemistry Vol. 343; p. 128437
• Main Authors: Kim, Sunbin, Shin, Weon-Sun
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.05.2021
• Subjects: Biological Availability; BSA-fucoidan conjugate; Chlamydomonas reinhardtii - chemistry; Digestion; Electrophoresis, Polyacrylamide Gel; Emulsifying Agents - chemistry; Emulsion property; Emulsions - chemistry; Emulsions - pharmacokinetics; Humidity; Lutein - chemistry; Lutein - pharmacokinetics; Lutein/zeaxanthin delivery; Maillard Reaction; Polysaccharides - chemistry; Polysaccharides - pharmacokinetics; Serum Albumin, Bovine - chemistry; Storage stability; Temperature; Zeaxanthins - chemistry; Maillard reaction; Emulsion property; Lutein/zeaxanthin delivery; BSA-fucoidan conjugate; Storage stability
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2020.128437

•Dry-heating of bovine serum albumin with fucoidan for 4 day formed covalent bonds.•BSA-fucoidan conjugates improved physical stability of emulsion.•BSA-fucoidan conjugates could not inhibit xanthophyll’s degradation.•Emulsion stabilized by BSA-fucoidan conjugates had improved stability at gastric phase.•BSA-fucoidan conjugates had high bioaccessibility of bioactive compounds. The effective delivery of bioactive compounds has recently been receiving attention. In this study, a conjugate with BSA and fucoidan synthesized via the Maillard reaction was confirmed through electrophoresis, the o-phthalaldehyde assay, and through changes in absorbance. Two moles of fucoidan were glycated with one mole of BSA at 60 °C and 79% relative humidity for 4 days. The droplet coated with B-F conjugate remained stable during storage at 4 and 25 °C and slightly increased only at 55 °C however, the droplet coated with intact BSA and B/F mixture significantly increased. L/Z were degraded about 82, 79, and 36% for 4, 25, and 55 °C, respectively, regardless of the type of emulsifier. Although the conjugates could not prevent the degradation of lutein and zeaxanthin during storage, they improved the stability of the emulsion and showed 4.20-fold and 1.32-fold higher bioaccessibility than intact BSA and B/F mixtures, respectively.