Isolation, purification, characterization, and antioxidant activity of low-molecular-weight polysaccharides from Sparassis latifolia

• Published in: International journal of biological macromolecules Vol. 137; pp. 1112 - 1120
• Main Authors: Duan, Guo-Liang, Yu, Xiao-bin
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.09.2019
• Subjects: Antioxidant activity; Biphenyl Compounds - chemistry; Free Radical Scavengers - chemistry; Free Radical Scavengers - isolation & purification; Fungal Polysaccharides - chemistry; Fungal Polysaccharides - isolation & purification; Hydroxyl Radical - chemistry; Isolation; Molecular Weight; Mycelium - chemistry; Picrates - chemistry; Polyporales - chemistry; Polysaccharide; Sparassis latifolia; Structure-function relationship; Isolation; Sparassis latifolia; Polysaccharide; Antioxidant activity; Structure-function relationship
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2019.06.177

Six polysaccharides (SF-FB11, SF-HW21, SF-CA31, SF-HA41, SF-FF51, and SF-FR61) of similar molecular weights (MW) (30–50 kDa) were extracted from the fermentation liquor, mycelia, and basidiomata of Sparassis latifolia by different methods. Structural analyses of these purified polysaccharides indicated that they were all branched, with a degree of branching (DB) ranging from 0.2 to 0.4. The polysaccharides exhibited strong scavenging activities for 2,2‑diphenyl‑1‑picrylhydrazyl and hydroxyl radicals. Their antioxidant activities were correlated to some extent with their composition but significantly with the DB. The polysaccharides with a low 1,3‑d‑glucose content and glucose: galactose ratio, or a low DB (0.2–0.3) had higher antioxidant activities. Additionally, the correlation between the extraction yield and antioxidant activities of the polysaccharides was assessed. The low-MW polysaccharides extracted by alkali treatments had both high yield and antioxidant activities. Additionally, both the yield and antioxidant activities were found to be influenced by the size, extraction method, and source (fermentation liquor, mycelia, or basidiomata) of the polysaccharides. These results indicate that polysaccharides isolated from S. latifolia have strong antioxidant activities closely associated with the polysaccharide structure and extraction method, thus providing a scientific basis for further use of these polysaccharides. •Alkali-extracted polysaccharides from Sparassis latifolia showed higher antioxidant activity.•The activity was correlated to degree of branching and monosaccharide composition.•Polysaccharides extracted from the stipe and base were more active than from the flabellae part.