Effect of Endothelin-1 on proliferation, migration and fibrogenic gene expression in human RPE cells

• Published in: Peptides (New York, N.Y. : 1980) Vol. 94; pp. 43 - 48
• Main Authors: Qin, Dong, Zhang, Li, Jin, Xuemin, Zhao, Zhaoxia, Jiang, Yanrong, Meng, Zijun
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.08.2017
• Subjects: Cell Line; Cell Movement; Cell Proliferation; Collagen Type I - secretion; Cytokines - secretion; Endothelin-1; Endothelin-1 - metabolism; Extracellular Matrix - metabolism; Fibronectin; Gene Expression Regulation; Humans; MAP Kinase Signaling System; Proliferative vitreoretinopathy; Retinal Pigment Epithelium - metabolism; Retinal Pigment Epithelium - physiology; Retinal Pigment Epithelium - secretion; Type I collagen; Vitreoretinopathy, Proliferative - metabolism; Type I collagen; Proliferative vitreoretinopathy; Fibronectin; Endothelin-1
• ISSN: 0196-9781; 1873-5169
• DOI: 10.1016/j.peptides.2017.06.004

•ET-1 promotes prolinferation, migration and the expression of type I collagen and fibronectin in human RPE cells.•Akt and Erk signaling pathways mediate the effect of ET-1 on proliferation, migration and the expression of collagen I and fibronectin in RPE cells.•ET-1 may play a vital role in the development of proliferative vitreoretinopathy. The pathology of the fibrotic proliferative vitreoretinopathy (PVR) membrane represents an excessive wound healing response characterised by cells’ proliferation, migration and secretion of extracellular matrix molecules (ECMs). Retinal pigment epithelial (RPE) cells are a major cellular component of the fibrotic membrane. Endothelin-1 (ET-1) has been reported to be involved in the development of PVR in vivo research. However, little is known about the role of ET-1 in RPE cells in vitro. In the present study, we investigated the role of ET-1 in the proliferation, migration and secretion of ECMs (such as type I collagen and fibronectin) in RPE cells in vitro. Our results illustrated that ET-1 promoted the proliferation, migration and secretion of ECMs through the protein kinase B (Akt) and extracellular signal-regulated kinase (Erk) signaling pathways in RPE cells in vitro. These findings strongly suggested that ET-1 may play a vital role in the development of PVR.