Removal of bisphenol A in aqueous solution using magnetic cross-linked laccase aggregates from Trametes hirsuta

• Published in: Bioresource technology Vol. 306; p. 123169
• Main Authors: Sadeghzadeh, Sadegh, Ghobadi Nejad, Zahra, Ghasemi, Shahnaz, Khafaji, Mona, Borghei, Seyed Mehdi
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.06.2020
• Subjects: Bisphenol A; Enzyme immobilization; Laccase; Magnetic cross-linked laccase aggregates; Bisphenol A; Enzyme immobilization; Magnetic cross-linked laccase aggregates; Laccase
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2020.123169

[Display omitted] •Magnetic nanoparticles synthesis and amino-functionalization.•Preparation of magnetic cross-linked laccase aggregates from Trametes hirsuta.•Easy recycling of magnetic cross-linked laccase aggregates.•Disability of laccase immobilization by cross-linked enzyme aggregates method.•Successful bisphenol A removal using magnetic cross-linked laccase aggregates. Enzymatic removal of Bisphenol A (BPA), acknowledged as an environmentally friendly approach, is a promising method to deal with hard degradable contaminants. However, the application of “enzymatic treatment” has been limited due to lower operational stability and practical difficulties associated with recovery and recycling. Enzyme immobilization is an innovative approach which circumvents these drawbacks. In this study, laccase from Trametes hirsuta was used for BPA removal. Amino-functionalized magnetic Fe3O4 nanoparticles were synthesized via the co-precipitation method followed by surface modification with (3-aminopropyl)trimethoxysilane (APTMS). The as-prepared nanoparticles were utilized for the immobilization of laccase with the magnetic cross-linked enzyme aggregates method (MCLEAs). Activity recovery of 27% was achieved, while no immobilized laccase was observed in the cross-linked enzyme aggregates method. The performance of immobilized laccase was measured by analyzing the degradation of BPA pollutant. The maximum removal efficiency of 87.3% was attained with an initial concentration of 60 ppm throughout 11 h.