Electrophoretic, Immunologic and Kinetic Characterization of Erythrocyte Pyruvate Kinase in the Basenji Dog with Pyruvate Kinase Deficiency

The electrophoretic mobility and the immunologic specificity of erythrocyte pyruvate kinase (PK) of the homozygous Basenji dog with PK deficiency were identical to those of normal M2-type PK isozyme seen in the white cell but not to those of the erythrocyte PK isozyme. Kinetic properties and stabili...

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Published inThe Tohoku Journal of Experimental Medicine Vol. 117; no. 2; pp. 179 - 185
Main Authors NAKASHIMA, KOJI, MIWA, SHIRO, SHINOHARA, KENJI, ODA, ETSUKO, TAJIRI, MITSUAKI, ABE, SHIGENOBU, ONO, JUNICHIRO, BLACK, JOHN A.
Format Journal Article
LanguageEnglish
Published Japan Tohoku University Medical Press 1975
Subjects
Animals
Basenji dog
Disease Models, Animal
Dog Diseases - enzymology
Dogs
Electrophoresis, Polyacrylamide Gel
Erythrocytes - enzymology
hemolytic anemia
Heterozygote
Homozygote
Immune Sera
isozyme
Liver - enzymology
Muscles - enzymology
Pyruvate Kinase - deficiency
Pyruvate Kinase - immunology
Pyruvate Kinase - isolation & purification
Spleen - enzymology
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Summary:The electrophoretic mobility and the immunologic specificity of erythrocyte pyruvate kinase (PK) of the homozygous Basenji dog with PK deficiency were identical to those of normal M2-type PK isozyme seen in the white cell but not to those of the erythrocyte PK isozyme. Kinetic properties and stability were also consistent with the M2-type PK isozyme. Defective PK in the homozygous red cell was due to the absence of the erythrocyte PK isozyme and the compensatory presence of M2-type PK isozyme, as seen in the severe classical type PK deficiency in man.
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ISSN:0040-8727
1349-3329
DOI:10.1620/tjem.117.179