Efficient and easy‐to‐use capturing three‐dimensional metagenome interactions with GutHi‐C

• Published in: iMeta Vol. 3; no. 5; pp. e227 - n/a
• Main Authors: Lu, Yu‐Xi, Yang, Jin‐Bao, Li, Chen‐Ying, Tian, Yun‐Han, Chang, Rong‐Rong, Kong, Da‐Shuai, Yang, Shu‐Lin, Wang, Yan‐Fang, Zhang, Yu‐Bo, Zhu, Xiu‐Sheng, Pan, Wei‐Hua, Kong, Si‐Yuan
• Format: Journal Article
• Language: English
• Published: Australia John Wiley and Sons Inc 01.10.2024; Wiley
• Subjects: Correspondence
• ISSN: 2770-596X; 2770-5986; 2770-596X
• DOI: 10.1002/imt2.227

Hi‐C can obtain three‐dimensional chromatin structure information and is widely used for genome assembly. We constructed the GutHi‐C technology. As shown in the graphical , it is a highly efficient and quick‐to‐operate method and can be widely used for human, livestock, and poultry gut microorganisms. It provides a reference for the Hi‐C methodology of the microbial metagenome. DPBS, Dulbecco's phosphate‐buffered saline; Hi‐C, high‐through chromatin conformation capture; LB, Luria‐Bertani; NGS, next‐generation sequencing; PCR, polymerase chain reaction; QC, quality control.