Systematic characterization of chemical constituents in Mahuang decoction by UHPLC tandem linear ion trap‐Orbitrap mass spectrometry coupled with feature‐based molecular networking

Comprehensive characterization of traditional Chinese medicine prescriptions has long been a hurdle due to the chemical complexity and the lack of analytical tools. Mahuang decoction is a well‐known traditional Chinese medicine prescription widely used for sweating and relieving the exterior, reliev...

Full description

Saved in:
Bibliographic Details
Published inJournal of separation science Vol. 44; no. 14; pp. 2717 - 2727
Main Authors Chen, Ling, Yao, Changliang, Li, Jiayuan, Wang, Jing, Yao, Shuai, Shen, Shijie, Yang, Lin, Zhang, Jianqing, Wei, Wenlong, Bi, Qirui, Guo, De‐an
Format Journal Article
LanguageEnglish
Published Germany Wiley Subscription Services, Inc 01.07.2021
Subjects
Alkaloids - analysis
Asthma
Chinese medicine
Chromatography, High Pressure Liquid - methods
Data processing
Drugs, Chinese Herbal - chemistry
feature‐based molecular networking
Flavanones - analysis
Flavones - analysis
Gas Chromatography-Mass Spectrometry - methods
Glycosides
Glycosides - analysis
Ions
linear ion trap‐Orbitrap
Liquid chromatography
Mahuang decoction
Mass spectrometry
Mass Spectrometry - methods
Medicine, Chinese Traditional
Procyanidins
Protein Interaction Maps
Saponins
Scientific imaging
Spectroscopy
Traditional Chinese medicine
traditional Chinese medicine
Mahuang decoction
linear ion trap-Orbitrap
feature-based molecular networking
Online AccessGet full text

Cover

More Information
Summary:Comprehensive characterization of traditional Chinese medicine prescriptions has long been a hurdle due to the chemical complexity and the lack of analytical tools. Mahuang decoction is a well‐known traditional Chinese medicine prescription widely used for sweating and relieving the exterior, relieving cough and asthma, but it was insufficiently chemically scrutinized. In this study, the chemical component information of Mahuang decoction was investigated by ultrahigh‐performance liquid chromatography tandem linear ion trap‐Orbitrap mass spectrometry. A new data processing tool, feature‐based molecular networking, was introduced for grouping and elucidating the compounds. In this way, 156 chemical components were identified or tentatively characterized, including alkaloids, triterpenoid saponins, flavanone‐O‐glycosides, flavone‐C‐glycosides, and procyanidins. Thus, this research provides a solid foundation for further development of Mahuang decoction, and the adopted method is expected to be applied to other traditional Chinese medicine prescriptions.
Bibliography:Both the authors contributed equally to this work.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.202100121