Quantitation of the ABO system antigens by means of isoprecipitating antisera

• Published in: British journal of haematology Vol. 30; no. 1; p. 75
• Main Authors: Pintera, J, Vacl, J, Hrncírová, D, Kurtinová, J, Zelená, E
• Format: Journal Article
• Language: English
• Published: England 01.05.1975
• Subjects: ABO Blood-Group System; Chemical Precipitation; Counterimmunoelectrophoresis; Female; Humans; Isoantibodies - analysis; Male; Precipitins - isolation & purification
• ISSN: 0007-1048
• DOI: 10.1111/j.1365-2141.1975.tb00520.x

A quantitative method for the determination of blood group specific substances A and B on red blood cells was developed, using them to absorb isoprecipitating antisera anti-A and anti-B, followed by estimation of the residual antibody by means of a counterimmunoelectrophoretic technique with standard solutions of A and B substances prepared from saliva. The method was tested on a population of A2B donors. The mean amount of A substance in A2B RBC was 131 X 10(-12) +/- 217 X 10(-12) u/cell in the population of 95 donors. The A substance thus determined agreed significantly with values obtained by classical haemagglutinating titration. The mean amount of B substance in A2B RBC was 222 X 10(-12) +/- 178 X 10(-12) m/cell. The amounts of both the A and B substances were not correlated on the same RBC. Several peaks of the distribution curve of both substances confirmed the non-homogeneity of the populations, there being a wide standard deviation presumably due to differences in the antigen content of different subgroups.