Pharmacokinetic study and the extraction efficiency of bulk and precipitated ibuprofen‐imprinted polymer sorbents for gas chromatography and gas chromatography‐mass spectrometry ibuprofen bio‐analysis

• Published in: Journal of separation science Vol. 46; no. 13; pp. e2201031 - n/a
• Main Authors: Ebrahimi, Bahram, Mozaffari, Samaneh, Mohammadiazar, Sirwan
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.07.2023
• Subjects: Acetonitrile; Analgesics; Blood plasma; Bulk polymerization; Chemical synthesis; Chromatography; Fourier transforms; Gas chromatography; Ibuprofen; Imprinted polymers; Infrared analysis; kinetic study; Mass spectrometry; Mixtures; molecularly imprinted polymer; Nonsteroidal anti-inflammatory drugs; pharmacokinetic study; Pharmacokinetics; Photomicrographs; Plasma; Polymers; precipitation polymerization; Sorbents; Toluene; pharmacokinetic study; molecularly imprinted polymer; ibuprofen; kinetic study; precipitation polymerization
• ISSN: 1615-9306; 1615-9314
• DOI: 10.1002/jssc.202201031

Bulk and precipitation polymerization methods were used to prepare ibuprofen‐molecularly imprinted polymers. Molecularly imprinted polymer‐bulk and ‐precipitation were synthesized in acetonitrile, likewise molecularly imprinted polymer‐bulk (mixture) and molecularly imprinted polymer‐precipitation (mixture) in a mixture of acetonitrile/toluene (75:25 v/v). N2 adsorption‐desorption analysis data revealed that molecularly imprinted polymer‐precipitation (mixture) has the highest specific surface area (200.74 m2/g). The surface chemistry and morphology of the synthesized sorbents were investigated by Fourier‐transform infrared analysis and scanning electron microscope micrographs respectively. The prepared sorbents in the mixture of solvents were used in a dispersive solid‐phase extraction process for selective extraction and pre‐concentration of ibuprofen from urine and human plasma samples. The detection limits were 62.91 and 7.89 ng/ml using molecularly imprinted polymer‐bulk (mixture) and molecularly imprinted polymer‐precipitation (mixture), respectively. Also, the sorbents showed selective behavior to extract ibuprofen in the presence of naproxen, fenoprofen, and ketoprofen. Overall, the results showed that the precipitation method in the mixture of acetonitrile/toluene resulted in the preparation of a sorbent with the highest extraction efficiency. Furthermore, a pharmacokinetic study was done. The maximum plasma concentration, the time required for maximum plasma concentration, and plasma half‐life were 28.95 μg/ml, 2, and 2.39 h, respectively.