Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

• Published in: Electrophoresis Vol. 43; no. 1-2; pp. 82 - 108
• Main Author: Kašička, Václav
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.01.2022
• Subjects: Amino acids; Capillary electrochromatography; Capillary electrophoresis; Chemical reactions; Electrochromatography; Electrokinetics; Electromigration; Electrophoresis; Electrophoresis, Capillary; Isoelectric Focusing; Peptide Mapping; Peptides; Peptides - isolation & purification; Proteins; Proteins - analysis; Qualitative analysis; Review; Semiconductors; Proteins; Capillary electrochromatography; Review; Peptides; Capillary electrophoresis
• ISSN: 0173-0835; 1522-2683
• DOI: 10.1002/elps.202100243

The review provides a comprehensive overview of developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, microscale isolation, and physicochemical characterization of peptides from 2019 up to approximately the middle of 2021. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis, such as sample preparation, sorption suppression, EOF control, and detection, are presented. New developments in the individual CE and CEC methods are demonstrated and several types of their applications are shown. They include qualitative and quantitative analysis, determination in complex biomatrices, monitoring of chemical and enzymatic reactions and physicochemical changes, amino acid, sequence, and chiral analyses, and peptide mapping of proteins. In addition, micropreparative separations and determination of significant physicochemical parameters of peptides by CE and CEC methods are described.