Spatiotemporal Protein Expression Profiles of QSOX1 in the Murine Uterus, Placenta, and Embryo during Pregnancy

• Published in: Applied sciences Vol. 11; no. 21; p. 10151
• Main Authors: Lin, Hung-Shih, Lee, Robert Kuo-Kuang, Yang, Tsung-Hsien, Fang, Hsu-Wei, Li, Sheng-Hsiang
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.11.2021
• Subjects: Animals; Antibodies; Cardiac muscle; Cerebellum; Decidua; Diaphragm; embryo; Embryos; Epidermis; Laboratories; Labyrinth; Localization; maternal-fetal interface; Medulla oblongata; Mesencephalon; Muscles; Olfactory system; Oxidation; Placenta; Pons; Pregnancy; Protein expression; Proteins; quiescin sulfhydryl oxidase 1; Sperm; Spine; Sulfhydryl oxidase; tissue distribution; Uterus; Vagina; Variance analysis; Vomeronasal organ; United States > US; Taiwan
• ISSN: 2076-3417; 2076-3417
• DOI: 10.3390/app112110151

Quiescin Q6 sulfhydryl oxidase 1 (QSOX1) catalyzes the oxidation of the sulfhydryl group to disulfide bond and is widely expressed in various tissues. This study focuses on investigating QSOX1′s spatiotemporal and cellular protein expression profile of the pregnant uterus, placenta, and developing embryo during mouse pregnancy. Immunohistochemical staining was used to reveal the localization of QSOX1 protein, and HistoQuest was applied to quantify protein levels. The expression level of QSOX1 in the decidua and muscle cells of the pregnant uterus fluctuated dramatically during pregnancy. QSOX1 was ubiquitously expressed in the labyrinth, junction zone, and chorionic plate in the placenta. The quantitative analysis found that this protein was highly expressed in the spinal cord, lens, midbrain, cerebellum, medulla oblongata, and tooth of mouse embryos, followed by the heart, intercostal muscle, diaphragm, intermediate zone, extrinsic ocular muscle, spine, pons, epidermis, tongue, ganglion, vomeronasal organ, thoracic vertebrae, and thymus. Interestingly, QSOX1 was also markedly expressed in olfactory system tissues. This comprehensive spatiotemporal study of QSOX1 protein expression will provide a basis for further investigations of the QSOX1 physiological function in the pregnant uterus, placenta, and developing embryo.