Analysis of proteinuria using a commercial system for automated electrophoresis and isoelectric focusing

We describe an investigation of proteinuria using Pharmacia PhastSystemTM electrophoresis apparatus. The analysis of urinary proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of unconcentrated urine followed by silver staining took about 2 h and could clearly demonstr...

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Bibliographic Details
Published inAnnals of clinical biochemistry Vol. 25 ( Pt 3); p. 319
Main Authors Jackson, P J, Sampson, C J, Cooper, E H, Heney, D, Brocklebank, J T
Format Journal Article
LanguageEnglish
Published England 01.05.1988
Subjects
Autoanalysis
Bence Jones Protein - urine
Child
Diabetic Nephropathies - urine
Electrophoresis, Polyacrylamide Gel
Female
Humans
Isoelectric Focusing
Lupus Erythematosus, Systemic - urine
Male
Muscular Diseases - urine
Proteinuria - urine
Sodium Dodecyl Sulfate
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Summary:We describe an investigation of proteinuria using Pharmacia PhastSystemTM electrophoresis apparatus. The analysis of urinary proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) of unconcentrated urine followed by silver staining took about 2 h and could clearly demonstrate tubular dysfunction or glomerular damage in urines with a negative or only trace-positive dip-stick test for protein. In addition, we show the identification of urinary proteins by immunoblotting from SDS-PAGE gels and the characterisation of Bence-Jones proteins by isoelectric focusing (IEF) and immunoblotting.
ISSN:0004-5632
DOI:10.1177/000456328802500322