Novel vectors for retrotransposon-induced gene silencing in Magnaporthe oryzae

Here we report a novel system to induce RNA silencing in Magnaporthe oryzae using the retrotransposon MAGGY. The gene-silencing vector pSilent-MG carries a MAGGY variant with a unique cloning site in the 3' UTR region and a geneticin-resistance cassette as a selection marker. A gene-silencing a...

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Bibliographic Details
Published inJournal of general plant pathology : JGPP Vol. 77; no. 3; pp. 147 - 151
Main Authors Van Vu, Ba, Takino, Masako, Murata, Toshiki, Nakayashiki, Hitoshi
Format Journal Article
LanguageEnglish
Published Tokyo Springer Nature B.V 01.05.2011
Subjects
Cloning
Deoxyribonucleic acid
DNA
Gene expression
Plant pathology
Plant resistance
Ribonucleic acid
RNA
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Summary:Here we report a novel system to induce RNA silencing in Magnaporthe oryzae using the retrotransposon MAGGY. The gene-silencing vector pSilent-MG carries a MAGGY variant with a unique cloning site in the 3' UTR region and a geneticin-resistance cassette as a selection marker. A gene-silencing assay with a GFP gene revealed that pSilent-MG vectors induced gene silencing at a practical level. In the resulting GFP-silenced transformants, GFP siRNAs accumulated and DNA methylation increased in the GFP-expressing gene, implying that the vector could induce both transcriptional and post-transcriptional gene silencing.[PUBLICATION ABSTRACT]
ISSN:1345-2630
1610-739X
DOI:10.1007/s10327-011-0302-5