Purification and characterization of a novel toxin from Bipolaris sorokiniana, causing spot blotch of wheat and analysis of variability in the pathogen

Bipolaris sorokiniana, causal agent of spot blotch of wheat is a hemibiotrophic pathogen. This fungus produced a toxin in culture that induced necrosis not only in wheat but also in barley, sorghum and some weeds. The toxic compound purified by prep TLC from culture filtrate of virulent strain BS-75...

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Published inCereal research communications Vol. 42; no. 2; pp. 252 - 261
Main Authors Jahani, M, R. Aggarwal, S. Gupta, S. Sharma, P. Dureja
Format Journal Article
LanguageEnglish
Published Cham Akadémiai Kiadà 01.06.2014
Springer International Publishing
Springer
Subjects
Agriculture
Avena sativa
barley
bioassays
Bipolaris sorokiniana
Chemical properties
corn
culture filtrates
Cynodon dactylon
Diseases and pests
fungi
gas chromatography-mass spectrometry
GC-MS
high performance liquid chromatography
leaves
Life Sciences
Methods
Mycotoxins
necrosis
nuclear magnetic resonance spectroscopy
pathogens
Pathology
pathotoxin
Phalaris minor
Plant Breeding/Biotechnology
Plant Genetics and Genomics
Plant Physiology
sesquiterpenoids
toxic substances
variability
virulent strains
weeds
Wheat
GC-MS
pathotoxin
variability
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Summary:Bipolaris sorokiniana, causal agent of spot blotch of wheat is a hemibiotrophic pathogen. This fungus produced a toxin in culture that induced necrosis not only in wheat but also in barley, sorghum and some weeds. The toxic compound purified by prep TLC from culture filtrate of virulent strain BS-75, characterized using NMR and GC-MS techniques, was identified to be ‘Bipolaroxin’, which is a first report of its production by B. sorokiniana infecting wheat. It is a bicyclical sesquiterpene belonging to family Eremophilane. Besides producing necrotic lesions on wheat, toxin (30 ng/ml) caused necrotic lesions on barley, maize, sorghum, Phalaris minor, Avena sativa and Cynodon dactylon as studied using leaf infiltration bioassay. Qualitative and quantitative differences among the pathogenically variable isolates were observed with respect to toxin production by TLC and HPLC. Different isolates produced bipolaroxin in the range of 0.05 µg/ml (BS-41) to 1.4 µg/ml (BS-75).
Bibliography:http://dx.doi.org/10.1556%2FCRC.2013.0053
ISSN:0133-3720
1788-9170
DOI:10.1556/CRC.2013.0053