Preparation of pyridyl disulfide-functionalized magnetic nanoparticles and application in traceless isolation of thiol-containing proteins

Thiol-reactive group - pyridyl disulfide functionalized magnetic nanoparticles were prepared via co-precipitation following functionalization by stöber method. The particles were characterized and employed to fast and selective conjugate and isolate protein with free thiol from mixed proteins under...

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Published inMaterials letters Vol. 186; pp. 386 - 389
Main Authors Liu, Changgeng, Du, Xiufang, He, Qingke, Zhu, Guangqian, Zhang, Lixia, Zhu, Junling, Xiao, Shangma, Zeng, Ying
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 01.01.2017
Elsevier BV
Subjects
Conjugates
Coprecipitation
Disulfides
Isolation
Magnetic materials
Materials science
Materials selection
Nanoparticle
Nanoparticles
Precipitation
Proteins
Pyridyl disulfide
Studies
Thiol-containing protein
Thiols
Traceless
SDS-PAGE
NMR
MRI
Nanoparticle
Traceless
FT-IR
Isolation
Thiol-containing protein
Magnetic materials
TCEP
MNP
Pyridyl disulfide
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Summary:Thiol-reactive group - pyridyl disulfide functionalized magnetic nanoparticles were prepared via co-precipitation following functionalization by stöber method. The particles were characterized and employed to fast and selective conjugate and isolate protein with free thiol from mixed proteins under mild condition. And the released protein has the original sulfhydryl group without any unnecessary modification. •Preparation of the pyridyl disulfide functionalized magnetic particles.•Characterization of the synthesized magnetic particles.•Fast isolation of thiol-containing protein followed by analysis.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0167-577X
1873-4979
DOI:10.1016/j.matlet.2016.10.044