A phenytoin assay using dried blood spot samples suitable for domiciliary therapeutic drug monitoring

A commercially available substrate-labelled fluorescent immunoassay procedure has been modified to create a simple rapid method for the determination of capillary phenytoin concentration in dried filter paper blood spots of patients on phenytoin therapy. The specimens are collected and dispatched to...

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Bibliographic Details
Published inAnnals of clinical biochemistry Vol. 21 ( Pt 6); p. 519
Main Authors Coombes, E J, Gamlen, T R, Batstone, G F, Leigh, P N
Format Journal Article
LanguageEnglish
Published England 01.11.1984
Subjects
Capillaries
Drug Stability
Female
Hematocrit
Humans
Male
Phenytoin - blood
Phenytoin - therapeutic use
Specimen Handling
Spectrometry, Fluorescence - methods
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Summary:A commercially available substrate-labelled fluorescent immunoassay procedure has been modified to create a simple rapid method for the determination of capillary phenytoin concentration in dried filter paper blood spots of patients on phenytoin therapy. The specimens are collected and dispatched to the laboratory by the patient, thus domiciliary monitoring of phenytoin therapy can take place. The technique was validated by comparing the phenytoin results of simultaneously collected capillary dried blood spots and conventional plasma samples. The technique offers a convenient method for overcoming many of the practical problems of monitoring phenytoin therapy in epilepsy.
ISSN:0004-5632
DOI:10.1177/000456328402100615