Adsorption of human serum albumin onto polypyrrole powder and polypyrrole-silica nanocomposites

• Published in: Synthetic metals Vol. 102; no. 1; pp. 1419 - 1420
• Main Authors: Azioune, Ammar, Pech, Karine, Saoudi, Bachir, Chehimi, Mohamed M., McCarthy, Gerard P., Armes, Steven P.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Lausanne Elsevier B.V 01.06.1999; Amsterdam Elsevier Science; New York, NY
• Subjects: Adsorption isotherms; Applied sciences; Biological and medical sciences; Biological materials; Composites; Exact sciences and technology; Forms of application and semi-finished materials; Fundamental and applied biological sciences. Psychology; In solution. Condensed state. Thin layers; Interfaces (materials); Molecular biophysics; Nanostructured materials; pH effects; Physico-chemical properties of biomolecules; Polymer industry, paints, wood; Polypyrroles; Silica; Synthesis (chemical); Technology of polymers; Ultraviolet spectroscopy; X ray photoelectron spectroscopy
• ISSN: 0379-6779; 1879-3290
• DOI: 10.1016/S0379-6779(98)00982-5

Adsorption of human serum albumin (HSA) onto polypyrrole powder and polypyrrole-silica nanocomposite is investigated at pH 7.4 and RT using UV spectroscopy. the nanocomposite is more effective than the powder in adsorbing HSA (147 and 63 mg/g, respectively). XPS was used to detect characteristic features of the adsorbed HSA and to construct adsorption isotherms. These latter were in fair agreement with those obtained via UV. Polypyrrole-silica is adsorptive towards HSA at pH 7.4 by contrast with the quasi-zero adsorption previously obtained with the DNA adsorbate (Saoudi et al., J. Colloid Interface Sci. 1997).