Detection of Listeria monocytogenes in raw whole milk for human consumption in Colombia by real-time PCR

The aim of our work was to detect Listeria monocytogenes in raw whole milk from five municipalities of Boyacá-Colombia using real-time PCR. A PCR hybridization probe format was used to analyze the presence of L. monocytogenes, using specific primers to amplify a 149 bp fragment from the metalloprote...

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Bibliographic Details
Published inFood control Vol. 20; no. 4; pp. 430 - 432
Main Authors Vanegas, Maria Consuelo, Vásquez, Elizabeth, Martinez, Aida Juliana, Rueda, Adriana M.
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.04.2009
Subjects
bacterial contamination
food contamination
food pathogens
Listeria monocytogenes
microbial detection
milk analysis
milk composition
polymerase chain reaction
raw milk
Raw whole milk
Real-time PCR
real-time polymerase chain reaction
whole milk
Colombia
Real-time PCR
Listeria monocytogenes
Raw whole milk
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Summary:The aim of our work was to detect Listeria monocytogenes in raw whole milk from five municipalities of Boyacá-Colombia using real-time PCR. A PCR hybridization probe format was used to analyze the presence of L. monocytogenes, using specific primers to amplify a 149 bp fragment from the metalloprotease gene ( mpl). In this study on a total number of 81 samples, 21 gave positive results for L. monocytogenes by real-time PCR and 13 samples were positive by conventional method. These results indicate a high presence of this pathogen in this area of the country and that this method is considerably faster than current standard methods.
Bibliography:http://dx.doi.org/10.1016/j.foodcont.2008.07.007
ObjectType-Article-1
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ISSN:0956-7135
1873-7129
DOI:10.1016/j.foodcont.2008.07.007