X-Shaped ZIF-8 for Immobilization Rhizomucor miehei Lipase via Encapsulation and Its Application toward Biodiesel Production

This study presents a one-step encapsulation method for synthesizing X-shaped zeolitic imidazolate frameworks (ZIF-8) and immobilizing Rhizomucor miehei lipase (RML). We proved that the morphological structure of ZIF-8 had changed after immobilization with enhanced characterization using a field-emi...

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Bibliographic Details
Published inCatalysts Vol. 8; no. 3; p. 96
Main Authors Adnan, Miaad, Li, Kai, Xu, Li, Yan, Yunjun
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.03.2018
Subjects
Biodiesel fuels
biodiesel yield
Catalysts
Chemical reactions
Conversion
Electron microscopes
Encapsulation
encapsulation technique
Energy transmission
Enzymes
Fourier transforms
FTIR spectrometers
Fungi
Immobilization
Isooctane
Lipase
Metal-organic frameworks
Pore size
Porosity
Rhizomucor miehei lipase (RML)
Soybean oil
Soybeans
X ray powder diffraction
X-ray diffraction
X-shape ZIF-8
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Summary:This study presents a one-step encapsulation method for synthesizing X-shaped zeolitic imidazolate frameworks (ZIF-8) and immobilizing Rhizomucor miehei lipase (RML). We proved that the morphological structure of ZIF-8 had changed after immobilization with enhanced characterization using a field-emission scanning electron microscope, an energy-dispersive spectrometer, a transmission electron microscope, a Fourier transform infrared spectrometer, and powder X-ray diffraction. The surface area and pore size of the carrier were investigated before and after immobilization using Brunauer-Emmett-Teller and Barrett-Joyner-Halenda methods, respectively. RML@ZIF-8 exhibited high recovery activity of up to 2632%, representing a 26-fold increase in its free lipase. Encapsulated RML was used for biodiesel production from soybean oil in an isooctane system with a conversion yield of 95.6% under optimum conditions. The resulting reusability of the immobilized enzyme indicated no substantial decline in the conversion yield, which remained at 84.7% of the initial activity after 10 cycles. The stability and high performance of the immobilized enzyme are attributed to the harmony between RML and ZIF-8 based on the easy synthesis of ZIF-8 and the short time required to immobilize RML.
ISSN:2073-4344
2073-4344
DOI:10.3390/catal8030096