Photochemical binding of photoallergens to human serum albumin: a simple in vitro method for screening potential photoallergens

A simple procedure employing UV spectroscopy is described for testing the ability of chemicals to form covalent conjugates with proteins after irradiation with the appropriate wavelength of light. A range of known photoallergens of widely differing structure has been tested using this procedure; res...

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Bibliographic Details
Published inToxicology letters Vol. 24; no. 1; p. 1
Main Authors Barratt, M D, Brown, K R
Format Journal Article
LanguageEnglish
Published Netherlands 01.01.1985
Subjects
Allergens - metabolism
Humans
Methods
Photochemistry
Photosensitivity Disorders
Serum Albumin - metabolism
Spectrophotometry, Ultraviolet
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Summary:A simple procedure employing UV spectroscopy is described for testing the ability of chemicals to form covalent conjugates with proteins after irradiation with the appropriate wavelength of light. A range of known photoallergens of widely differing structure has been tested using this procedure; results of these experiments, together with evidence from the scientific literature, provide a correlation between compounds known to be photoallergens and their ability to form covalent conjugates with proteins on irradiation with the appropriate wavelength of light. The method is proposed as an in vitro screening procedure for potential photoallergens.
ISSN:0378-4274
DOI:10.1016/0378-4274(85)90131-6