Biotransformations of protoberberines in cell cultures of Dicentra spectablis

• Published in: Phytochemistry (Oxford) Vol. 46; no. 8; pp. 1359 - 1363
• Main Authors: Iwasa, Kinuko, Won Kim, Chong
• Format: Journal Article
• Language: English
• Published: OXFORD Elsevier Ltd 01.12.1997; Elsevier
• Subjects: Biochemistry & Molecular Biology; Bioconversions. Hemisynthesis; Biological and medical sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; Life Sciences & Biomedicine; Methods. Procedures. Technologies; Plant Sciences; Science & Technology; Dicentra spectablis; biotransformation; Fumariaceae; protoberberines; cell culture; Cell culture; Alkaloid; Molecular structure; Dicotyledones; Biotransformation; Angiospermae; Spermatophyta; Metabolic pathway; Metabolism
• ISSN: 0031-9422; 1873-3700
• DOI: 10.1016/S0031-9422(97)00500-1

A liquid chromatography-atmospheric pressure chemical ionization mass spectrometry procedure was applied to biotransformation experiments in cultured cells of Dicentra spectablis. The results show that the metabolism of protoberberines in D. spectablis closely parallels that found in Corydalis species. A liquid chromatography-atmospheric pressure chemical ionization mass spectrometry procedure was applied to biotransformation experiments in cultured cells of Dicentra spectablis. Interconversions of tetrahydroberberine and berberine and those of cis- and trans-13-methyltetrahydroprotoberberines and 13-methylberberine were demonstrated. The corresponding α- N-metho salts having the B/C- cis ring-junction were produced from cis- and trans-13-methyltetrahydroprotoberberines. These findings show that the metabolism of protoberberines in D. spectablis proceeds analogously to that found in Cordyalis species.