Subzonal fertilization of mouse round spermatids

• Published in: Science China. Life sciences Vol. 40; no. 2; pp. 152 - 158
• Main Author: 刘灵 孙青原 段崇文 刘辉 宋祥芬 钱菊汾 陈大元
• Format: Journal Article
• Language: English
• Published: China Springer Nature B.V 01.04.1997
• Subjects: electrofusion; Fertilization; mouse; Oocytes; Pseudopregnancy; round; spermatid; Spermatids; subzonal
• ISSN: 1674-7305; 1006-9305; 1869-1889; 1862-2798
• DOI: 10.1007/BF02882043

Mouse round spermatids were electrofused with homologous mature oocytes to examine the be-haviour of their nuclei within the ooplasm and the abilities of development. A single spermatid was injected in the perivitelline space of a mature oocyte and an electron fusion pulse was given. The best round spermatid-oocyte pairs (RS-O) fusion took place at 20-30 s AC (1 MHz, 50V/cm) followed by a single fusion DC pulse (3 700-3 800 V/cm, 25 μs) and another 30 s AC current. The total survival rate and fusion rate of RS-O were 89.0% (575/646) and 61. 9% (356/575), respectively. 49.2% (175/356) of fused oocytes developed to 2PN stage . The concentration of Ca2+ in the fusion medium produced no significant effect on the above targets. The 2PN development rate of the fused RS-O from the oocytes collected 14-16 h after hCG injection was higher than others. 32.6% (57/175) of the 2PN oocytes had fully developed spermatid (male) and oocyte (female) pronuclei. The rest spermatid-derived pronu-clei remained small in size