Network pharmacological analysis of the active molecules in Coeloglossum viride var. bacteatum and their anti-Alzheimer’s disease activity through restoration of energy metabolism and inhibition of inflammation

• Published in: Arabian journal of chemistry Vol. 16; no. 8; p. 105008
• Main Authors: Bai, Jin-Peng, Wang, Jun, Hu, Yang, Huang, Qin, Dai, Jing-Feng, Xiao, Guan-Li, Yu, Hui-Jing, Qin, Xiao-Yan, Lan, Rongfeng
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.08.2023; Elsevier
• Subjects: Astrocyte; BV2; GFAP; Glycolysis; MAP2; OXPHOS; PPI; OXPHOS; FCCP; IBA1; LPS; Aβ; MAP2; KEGG; BV2; CE; AD; PKM2; Nos2; GO; IL-4; 2-DG; Astrocyte; IL-1β; IL-6; GFAP; ECAR; TNF-α; Glycolysis; Rote/AA; ATP; OCR
• ISSN: 1878-5352; 1878-5379
• DOI: 10.1016/j.arabjc.2023.105008

CE attenuated LPS-induced inflammation and apoptosis in astrocytes, while also enhancing energy metabolism in BV2 cells, indicating its potential to be a promising anti-AD agent. [Display omitted] Coeloglossum viride var. bracteatum is an advanced tonic in Tibetan medicine with anti-inflammatory and neuroprotective effects. Therefore, its anti-AD effects and potential targets should be explored. Firstly, a network pharmacological analysis of its bioactive molecules and their targets related to AD was performed, and the anti-inflammatory and energy metabolism modulating effects of its extract (CE) were investigated in LPS-induced astrocytes and BV2 cellular models. Then, inflammatory factors levels, ATP content and real-time energy metabolism were measured by RT-qPCR and Seahorse extracellular flux assays, respectively. Finally, we established a molecule-target network, as well as a PPI network of CE-related targets with AD-related targets, and identified 178 nodes and 2317 edges, yielding key targets that may play an important role in the treatment of AD. CE attenuated LPS-induced inflammation and apoptosis in astrocytes, while also enhancing energy metabolism in BV2 cells. Mechanistically, CE inhibited LPS-induced enhancement of microglia glycolytic activity and improved energy metabolism by inhibiting the HIF-1α/PKM2 signaling axis. Thus, CE is a potential AD therapeutic agent with anti-inflammatory and energy metabolism modulating activities.