Use of luminescence-marked bacteria to assess copper bioavailability in malt whisky distillery effluent

Samples were taken from upstream, influent, effluent, and downstream locations of a whisky distillery in north east Scotland, and the concentration of inorganic pollutants determined using inductively coupled plasma mass spectrometry. The principal contaminant was found to be Cu, and three biolumine...

Full description

Saved in:
Bibliographic Details
Published inChemosphere (Oxford) Vol. 31; no. 5; pp. 3217 - 3224
Main Authors Paton, G.I., Palmer, G., Kindness, A., Campbell, C., Glover, L.A., Killham, K.
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 1995
Subjects
bacteria
bioassays
bioluminescence
copper
cytotoxicity
distillery effluent
Freshwater
gene transfer
indicator species
luciferase
luminescence
lux gene
Photobacterium phosphoreum
Pseudomonas fluorescens
reporter genes
water pollution
Scotland
British Isles, Scotland
Online AccessGet full text

Cover

More Information
Summary:Samples were taken from upstream, influent, effluent, and downstream locations of a whisky distillery in north east Scotland, and the concentration of inorganic pollutants determined using inductively coupled plasma mass spectrometry. The principal contaminant was found to be Cu, and three bioluminescence based microbial bioassays were carved out to assess the bioavailability of Cu. One assay involved standard use of a naturally luminescent marine bacterium and two involved use of genetically modified (luminescence-marked) terrestrial bacteria. Use of the luminescence-marked biosensors was found to be the most sensitive and reproducible, offering assessment of toxicity over a wide pH range.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:0045-6535
1879-1298
DOI:10.1016/0045-6535(95)00183-9