Comparison of six different methods for typing Pseudomonas aeruginosa strains isolated from bottled and well waters

Twenty-three strains of Pseudomonas aeruginosa isolated from bottled and well waters were biotyped, serotyped and examined by antimicrobial susceptibility, plasmid content, analysis of chromosomal DNA Eco RI ribopatterns (ribotyping) and by a PCR-based procedure. Phenotypic methods showed poor discr...

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Bibliographic Details
Published inWater research (Oxford) Vol. 31; no. 12; pp. 3169 - 3174
Main Authors Hernandez, Javier, Ferrus, Maria A., Hernandez, Manuel, Alonso, Jose L.
Format Journal Article
LanguageEnglish
Published Oxford Elsevier Ltd 01.12.1997
Elsevier Science
Subjects
AP-PCR
Applied sciences
Bacteriology
Biological and medical sciences
Biological and physicochemical phenomena
DNA
Epidemiology
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Microbiology
Natural water pollution
Pollution
Pseudomonas aeruginosa
RFLP
ribotyping
serotyping
water
Water treatment and pollution
Southern Europe
Europe
Spain
serotyping
DNA
ribotyping
Pseudomonas aeruginosa
AP-PCR
water
RFLP
Pseudomonadales
Drinking water
Ribotype
Typing
Feed water
Genotype
Method
Water spring
Phenotype
Restriction fragment length polymorphism
Random amplified polymorphic DNA
Molecular epidemiology
Bacteria
Pseudomonadaceae
Serotype
Biological contamination
Comparative study
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Summary:Twenty-three strains of Pseudomonas aeruginosa isolated from bottled and well waters were biotyped, serotyped and examined by antimicrobial susceptibility, plasmid content, analysis of chromosomal DNA Eco RI ribopatterns (ribotyping) and by a PCR-based procedure. Phenotypic methods showed poor discrimination power between strains, and plasmids were detected in 29% of isolates. Southern blot hydridisation analysis showed genetic diversity between isolates, and six different ribotypes were defined. Arbitrarily primed polymerase chain reaction (AP-PCR) analysis also differentiated between the strains with higher discriminatory power than ribotyping, since 13 different profiles were obtained. Both DNA-based methods are valuable alternatives to traditional typing systems for P. aeruginosa, and AP-PCR could be particularly useful for epidemiological studies.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0043-1354
1879-2448
DOI:10.1016/S0043-1354(97)00172-3