Speciation of organotin compounds in shellfish by liquid chromatography — fluorimetric detection

• Published in: Analytica chimica acta Vol. 443; no. 2; pp. 183 - 190
• Main Authors: González-Toledo, Encarna, Benzi, Mario, Compañó, Ramon, Granados, Mercè, Prat, Maria Dolors
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.09.2001; Elsevier
• Subjects: Analytical chemistry; Animal, plant and microbial ecology; Applied ecology; Biological and medical sciences; Bivalvia; Butyltin; Chemistry; Chromatographic methods and physical methods associated with chromatography; Ecotoxicology, biological effects of pollution; Exact sciences and technology; Fluorescence; Fundamental and applied biological sciences. Psychology; Marine; Marine and brackish environment; Marine organisms analysis; Other chromatographic methods; Phenyltin; Solid phase extraction; Fluorescence; Butyltin; Solid phase extraction; Marine organisms analysis; Phenyltin; Fluorescence detector; Biochemical analysis; Ecotoxicology; Shellfish; Liquid solid adsorption; Biological indicator; Liquid chromatography; Environmental control; Chemical enrichment; Chemical pollution; Multicomponent analysis; Tin Organic compounds; Environmental monitoring; Speciation; Organic stannane; Quantitative analysis
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/S0003-2670(01)01205-3

A method for the determination of butyltin and phenyltin species in marine organisms by liquid chromatography is described. Analytes are extracted from the samples using ethyl acetate and hydrochloric acid. A clean-up step based on a solid-phase extraction using a cation exchange bonded phase has been developed to remove coextracted substances from the ethyl acetate extract. Determination is carried out by gradient elution on a silica-based C 18 column with mobile phases consisting of methanol–water–acetic acid mixtures and using fluorimetric detection. The method, which allows the determination of the analytes at the ng g −1 level, has been applied to the analysis of diphenyltin, dibutyltin, triphenyltin and tributyltin in oyster and mussel tissues.