Cryoprotection of porcine cornea: a scanning electron microscopy study

Porcine corneas were frozen with Me2SO, glycerol, 1,2-propanediol and PEG-400. The effects of the range of concentrations (5% and 10%) and temperature regimen (1ºC/min and 5ºC/min) were investigated. The integrity of corneal endothelial cells was evaluated by scanning electron microscopy and trypan...

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Bibliographic Details
Published inVeterinární medicína Vol. 50; no. 5; pp. 219 - 224
Main Authors Neronov, A., Giurov, P., Cholakova, M., Dimitrova, M., Nikolova, E.
Format Journal Article
LanguageEnglish
Published Prague Czech Academy of Agricultural Sciences (CAAS) 01.05.2005
Czech Academy of Agricultural Sciences
Subjects
1,2-propanediol
Cornea
Electron microscopes
Endothelial cells
Endothelium
Freeze-thaw
Freezing
Glycerol
Integrity
me2so
peg-400
porcine cornea
Scanning electron microscopy
Thawing
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Summary:Porcine corneas were frozen with Me2SO, glycerol, 1,2-propanediol and PEG-400. The effects of the range of concentrations (5% and 10%) and temperature regimen (1ºC/min and 5ºC/min) were investigated. The integrity of corneal endothelial cells was evaluated by scanning electron microscopy and trypan blue staining. The presence of 5–10% PEG-400 in the protective medium was the most effective in minimizing changes in the integrity of the corneal endothelium during freezing-thawing procedures.
ISSN:0375-8427
1805-9392
DOI:10.17221/5618-VETMED