Cryoprotection of porcine cornea: a scanning electron microscopy study
Porcine corneas were frozen with Me2SO, glycerol, 1,2-propanediol and PEG-400. The effects of the range of concentrations (5% and 10%) and temperature regimen (1ºC/min and 5ºC/min) were investigated. The integrity of corneal endothelial cells was evaluated by scanning electron microscopy and trypan...
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Published in | Veterinární medicína Vol. 50; no. 5; pp. 219 - 224 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Prague
Czech Academy of Agricultural Sciences (CAAS)
01.05.2005
Czech Academy of Agricultural Sciences |
Subjects | |
Online Access | Get full text |
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Summary: | Porcine corneas were frozen with Me2SO, glycerol, 1,2-propanediol and PEG-400. The effects of the range of concentrations (5% and 10%) and temperature regimen (1ºC/min and 5ºC/min) were investigated. The integrity of corneal endothelial cells was evaluated by scanning electron microscopy and trypan blue staining. The presence of 5–10% PEG-400 in the protective medium was the most effective in minimizing changes in the integrity of the corneal endothelium during freezing-thawing procedures. |
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ISSN: | 0375-8427 1805-9392 |
DOI: | 10.17221/5618-VETMED |