Bioconversion of 2-cyanopyrazine to 5-hydroxypyrazine-2-carboxylic acid with Agrobacterium sp. DSM 6336

5-Hydroxypyrazine-2-carboxylic acid, a versatile building block for the synthesis of new antituberculous agents, was prepared by whole-cell biotransformation from 2-cyanopyrazine via pyrazinecarboxylic acid using Agrobacterium sp. DSM 6336. By developing a fermentation process for this two-enzyme-st...

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Bibliographic Details
Published inApplied microbiology and biotechnology Vol. 48; no. 2; pp. 174 - 176
Main Authors WIESER, M, HEINZMANN, K, KIENER, A
Format Journal Article
LanguageEnglish
Published Berlin Springer 01.08.1997
Springer Nature B.V
Subjects
Agrobacterium
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Biotransformation
Carboxylic acids
Fermentation
Fundamental and applied biological sciences. Psychology
Gram-negative bacteria
Health aspects
Methods. Procedures. Technologies
Microbiological chemistry
Pharmaceutical chemistry
Physiological aspects
Pyridazine
Switzerland
Enzymatic synthesis
Agrobacterium
Hydroxylation
Biotransformation
Microorganism culture
Pyrazine derivatives
Bacteria
Rhizobiaceae
Fermentation
Optimization
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Summary:5-Hydroxypyrazine-2-carboxylic acid, a versatile building block for the synthesis of new antituberculous agents, was prepared by whole-cell biotransformation from 2-cyanopyrazine via pyrazinecarboxylic acid using Agrobacterium sp. DSM 6336. By developing a fermentation process for this two-enzyme-step bioconversion, a product concentration of 286mM (40g/l) was obtained. After the isolation method had been optimized the total yield was 80%.[PUBLICATION ABSTRACT]
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0175-7598
1432-0614
DOI:10.1007/s002530051034