DNA adducts in earthworms exposed to a contaminated soil

• Published in: Soil biology & biochemistry Vol. 29; no. 3; pp. 721 - 724
• Main Authors: Walsh, P., El Adlouni, C., Nadeau, D., Fournier, M., Coderre, D., Poirier, G.G.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Oxford Elsevier Ltd 01.03.1997; New York, NY Elsevier Science
• Subjects: Agronomy. Soil science and plant productions; Animal, plant and microbial ecology; Applied ecology; Biological and medical sciences; Ecotoxicology, biological effects of pollution; Effects of pollution and side effects of pesticides on protozoa and invertebrates; Fundamental and applied biological sciences. Psychology; Soil and water pollution; Soil science; Canada; North America; America; Quebec; Toxicity; Chromatogram; Postlabeling; Soil pollution; Carcinogenesis; Oligochaeta; Chemical pollution; Earthworm; Soil fauna; Toxicogenetics; Molecular adduct; Phosphorus Isotopes; Ecotoxicology; Radiolabelling; Biological indicator; Polycyclic aromatic compound; Annelida; Lumbricidae; Macrofauna; DNA; Useful animal; Pollutant effect; Eisenia fetida; Invertebrata; Chemical contamination
• ISSN: 0038-0717; 1879-3428
• DOI: 10.1016/S0038-0717(96)00026-0

DNA adducts were quantified by 32P-postlabelling on the earthworm Eisenia fetida after an exposure to a polycyclic aromatic hydrocarbon (PAH)-contaminated soil sample. The pattern of adduct spots found on the chromatograms are consistent with the presence of bulky DNA adducts originating from PAH metabolites. After 1 or 2 weeks of exposure, amounts of DNA adducts in exposed worms (34.4 and 31.3 adducts per 10 9 nucleotides, respectively) were higher than those found in the unexposed control worms (16.5 adducts per 10 9 nucleotides).