Curcumin inhibits classical swine fever virus replication by interfering with lipid metabolism
•Curcumin and curcuminoids inhibits CSFV replication.•Curcumin decreases the lipid accumulation by regulating FASN, lipid droplets and ATF6.•Curcumin promotes innate immunity after viral infection. Although previous reports have shown that Curcumin inhibits many viruses, including some important mem...
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Published in | Veterinary microbiology Vol. 259; p. 109152 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Amsterdam
Elsevier B.V
01.08.2021
Elsevier BV |
Subjects | |
Online Access | Get full text |
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Summary: | •Curcumin and curcuminoids inhibits CSFV replication.•Curcumin decreases the lipid accumulation by regulating FASN, lipid droplets and ATF6.•Curcumin promotes innate immunity after viral infection.
Although previous reports have shown that Curcumin inhibits many viruses, including some important members of different genera of Flaviviridae family (Japanese encephalitis virus, dengue virus and hepatitis C virus), the antiviral activity of curcumin against Classical swine fever virus (CSFV), which belongs to Pestivirus genus, is still unclear. In this study, we found that curcumin inhibited CSFV replication in a dose-dependent manner, but had no effect on virus adsorption and entry. Furthermore, the results showed that curcumin inhibited the expression of FASN, one of the key enzymes of fatty acid synthesis pathway, thereby, causing the reduction of the production of LDs upon infection. To this end, we detected transcription factor 6 (ATF6), the key factor of regulating lipid metabolism along with other related molecules (CHOP and GPR78) and found that curcumin significantly impaired the gene synthesis of ATF6, while CSFV infection promoted ATF6 expression. Therefore, it is confirmed that curcumin inhibited CSFV replication by interfere lipid metabolism. In addition, our subsequent studies found that curcumin played an antiviral role by promoting the innate immune independent of NF-κB signaling pathway. Taken together, our finding highlights that curcumin is a potential candidate drug against CSFV for controlling CSF. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0378-1135 1873-2542 |
DOI: | 10.1016/j.vetmic.2021.109152 |