Antigens of infectious laryngotracheitis herpesvirus defined by monoclonal antibodies

• Published in: Archives of virology Vol. 115; no. 3/4; pp. 147 - 162
• Main Authors: York, J.J, Sonza, S, Brandon, M.R, Fahey, K.J
• Format: Journal Article
• Language: English
• Published: Austria 01.01.1990
• Subjects: Animals; Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - immunology; Antigens, Viral - immunology; Cells, Cultured; Enzyme-Linked Immunosorbent Assay; epitopes; Fluorescent Antibody Technique; glycoproteins; Glycoproteins - immunology; Herpesvirus 1, Gallid - drug effects; Herpesvirus 1, Gallid - immunology; Iltovirus; Immunoblotting; Mice; Mice, Inbred BALB C; monoclonal antibodies; Neutralization Tests; surface antigens; Tunicamycin - pharmacology; viral antigens
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/BF01310527

Monoclonal antibodies to glycoprotein and protein antigens of infectious laryngotracheitis virus (ILTV) were divided into five groups on the basis of their reactivity in immunofluorescence and Western blotting. Group I antibodies recognised a single band of 60 k and Group II antibodies recognised bands of 205, 160, 115, 90 and 85 k in Western blotting. In immunofluorescence both these groups of antibodies reacted with antigens located in the cytoplasm of fixed virus-infected cells and they also reacted with unfixed cells, suggesting that these antigens are on the surface of virus-infected cells. While Group I monoclonal antibodies did not react with extracts of tunicamycin-treated cells, some Group II antibodies recognised bands of decreased molecular weight compared to those present in untreated cells. The reactivity of the Group II antibodies with extracts of tunicamycin-treated cells suggested that they recognised at least three different epitopes which was confirmed by ELISA additivity assays. Monoclonal antibodies of Group III, Group IV and Group V recognised several low molecular weight proteins from 45 to 24 k. Immunofluorescence studies showed that these were nuclear and cytoplasmic antigens that were not present on the surface of virus-infected cells.