A New Nanogold-Labeled Immunoresonance Scattering Spectral Probe for Determination of Trace IgG
A kind of 9 nm gold nanoparticles was prepared with the trisodium citrate and used to label goat anti‐human IgG to obtain an IgG immunoresonance scattering spectral probe. In pH 5.8 buffer solution and in the presence of polyethylene glycol (PEG), the immune reaction between gold‐labeled goat anti‐h...
Saved in:
Published in | Chinese journal of chemistry Vol. 25; no. 9; pp. 1282 - 1287 |
---|---|
Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Weinheim
WILEY-VCH Verlag
01.09.2007
WILEY‐VCH Verlag |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | A kind of 9 nm gold nanoparticles was prepared with the trisodium citrate and used to label goat anti‐human IgG to obtain an IgG immunoresonance scattering spectral probe. In pH 5.8 buffer solution and in the presence of polyethylene glycol (PEG), the immune reaction between gold‐labeled goat anti‐human IgG and IgG took place, and the resonance scattering intensity at 580 nm (I580 nm) was enhanced greatly. The enhanced intensity ΔIRS is proportional to the IgG concentration from 1.3 to 1.5×103 ng·mL−1, with a detection limit of 0.78 ng·mL−1. This assay showed high sensitivity and good selectivity for quantitative determination of IgG in human serum, with satisfactory results. |
---|---|
Bibliography: | the National Natural Science Foundation of China - No. 20667001, 20365001 the Foundation of New Century Ten-Hundred-Thousand Talents of Guangxi ark:/67375/WNG-L6JM9JLN-F ArticleID:CJOC200790238 Natural Science Foundation of Guangxi - No. 2060213 istex:4A3B70756E3BDD2CDB42752110FED2BA31FF7907 |
ISSN: | 1001-604X 1614-7065 |
DOI: | 10.1002/cjoc.200790238 |