A New Nanogold-Labeled Immunoresonance Scattering Spectral Probe for Determination of Trace IgG

A kind of 9 nm gold nanoparticles was prepared with the trisodium citrate and used to label goat anti‐human IgG to obtain an IgG immunoresonance scattering spectral probe. In pH 5.8 buffer solution and in the presence of polyethylene glycol (PEG), the immune reaction between gold‐labeled goat anti‐h...

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Bibliographic Details
Published inChinese journal of chemistry Vol. 25; no. 9; pp. 1282 - 1287
Main Authors Jiang, Zhi-Liang, Li, Yan, Sun, Shuang-Jiao, Chen, Bing
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 01.09.2007
WILEY‐VCH Verlag
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Summary:A kind of 9 nm gold nanoparticles was prepared with the trisodium citrate and used to label goat anti‐human IgG to obtain an IgG immunoresonance scattering spectral probe. In pH 5.8 buffer solution and in the presence of polyethylene glycol (PEG), the immune reaction between gold‐labeled goat anti‐human IgG and IgG took place, and the resonance scattering intensity at 580 nm (I580 nm) was enhanced greatly. The enhanced intensity ΔIRS is proportional to the IgG concentration from 1.3 to 1.5×103 ng·mL−1, with a detection limit of 0.78 ng·mL−1. This assay showed high sensitivity and good selectivity for quantitative determination of IgG in human serum, with satisfactory results.
Bibliography:the National Natural Science Foundation of China - No. 20667001, 20365001
the Foundation of New Century Ten-Hundred-Thousand Talents of Guangxi
ark:/67375/WNG-L6JM9JLN-F
ArticleID:CJOC200790238
Natural Science Foundation of Guangxi - No. 2060213
istex:4A3B70756E3BDD2CDB42752110FED2BA31FF7907
ISSN:1001-604X
1614-7065
DOI:10.1002/cjoc.200790238