Antibody Against N-terminal Domain of Phospholipid Scramblase 1 Induces Apoptosis in Colorectal Cancer Cells Through the Intrinsic Apoptotic Pathway

• Published in: Chemical biology & drug design Vol. 84; no. 1; pp. 36 - 43
• Main Authors: Chen, Chun-Yu, Chen, Jinn-Shiun, Chou, Yeh-Pin, Kuo, Yung-Bin, Fan, Chung-Wei, Chan, Err-Cheng
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.07.2014
• Subjects: Antibodies - pharmacology; apoptosis; Apoptosis - drug effects; caspase; Caspase 3 - metabolism; Caspase 8 - metabolism; Caspase 9 - metabolism; Cell Line, Tumor; Colon - drug effects; Colon - enzymology; Colon - pathology; colorectal cancer; Colorectal Neoplasms - drug therapy; Colorectal Neoplasms - enzymology; Colorectal Neoplasms - pathology; Humans; Molecular Targeted Therapy; phospholipid scramblase 1; Phospholipid Transfer Proteins - chemistry; Phospholipid Transfer Proteins - metabolism; Protein Structure, Tertiary - drug effects; Rectum - drug effects; Rectum - enzymology; Rectum - pathology; phospholipid scramblase 1; apoptosis; colorectal cancer; caspase
• ISSN: 1747-0277; 1747-0285
• DOI: 10.1111/cbdd.12347

Phospholipid scramblase 1 involve in biological processes including phospholipid movement, proliferation, and apoptosis. Treatment with an antiphospholipid scramblase 1 antibody (NP1) has been demonstrated to inhibit cell proliferation in colorectal cancer. This study aimed to explore the role of NP1 treatment in the apoptosis of colorectal cancer cells. Results showed that NP1 treatment significantly increases the apoptosis of colorectal cancer cells via the activation of caspase 8, caspase 9, and caspase 3. Moreover, pretreatment with a caspase 8 inhibitor did not fully prevent the apoptotic effects of NP1. Taken together, these data indicate NP1 induces cell apoptosis primary through the intrinsic apoptotic pathway. NP1 may serve as a potential therapeutic agent. Treatment with an antiphospholipid scramblase 1 antibody (NP1) inhibited cell proliferation in colorectal cancer cell line. NP1 induced cell apoptosis primary through the intrinsic apoptotic pathway.