Cryopreservation of cattle semen using coconut water extender with different glycerol concentrations

• Published in: Asian Pacific Journal of Reproduction Vol. 6; no. 6; pp. 279 - 282
• Main Authors: El-Sheshtawy, Reda, El-Nattat, Walid, Ali, Gamal Abdel
• Format: Journal Article
• Language: English
• Published: Medknow Publications and Media Pvt. Ltd 01.11.2017; Animal Reproduction and Al Department, Veterinary Division, National Research Centre, Dokki, Giza, Egypt; Wolters Kluwer Medknow Publications
• Subjects: Additives; Beef cattle; Cattle; Coconut water; Freeze thawing; Fructose; Glycerol; Monosaccharides; Semen; Water; Glycerol; Coconut water; Freeze thawing; Semen
• ISSN: 2305-0500; 2305-0519
• DOI: 10.4103/2305-0500.217343

Objective:To investigate the effect of coconut water with a lone concentration and different concentrations of glycerol on chilled and cryopreserved cattle semen characteristics.Methods: Semen was collected from five mature cattle bulls, at weekly intervals for 5 weeks. The ejaculates were pooled and evaluated for dilution processing. Tris citrate egg yolk fructose was used as control treatment for semen, while 50% (V/V) coconut water, 25% (V/V) bi-distilled water and 25% (V/V, 5% anhydrous monosodium citrate) to 20 mL egg yolk and three different concentrations of glycerol (4%, 6% and 8%) were used as coconut water (CW)-glycerol-yolk extenders (CWCG-4, CWCG-6 and CWCG-8). Extended semen was cooled and cryopreserved. Sperm motility%, sperm membrane integrity%, normal acrosome%, live sperm% and total sperm abnormalities% were recorded after equilibrium and after freeze-thawing.Results:The addition of 4% glycerol to coconut water enriched media (CWCG-4) revealed the most effective addition of glycerol on all parameters after equilibrium and after freeze-thawing.Conclusions:Coconut water enriched media with 4% glycerol addition is safe to be used as an extender in bull semen preservation because it is a sterile liquid. So, it can be used without addition of antibiotics to the extender, as antibiotics have to some extent hazardous effect on spermatozoa.