β-cell deletion of the PKm1 and PKm2 isoforms of pyruvate kinase in mice reveals their essential role as nutrient sensors for the KATP channel

• Published in: eLife Vol. 11
• Main Authors: Foster, Hannah R, Ho, Thuong, Potapenko, Evgeniy, Sdao, Sophia M, Huang, Shih Ming, Lewandowski, Sophie L, VanDeusen, Halena R, Davidson, Shawn M, Cardone, Rebecca L, Prentki, Marc, Kibbey, Richard G, Merrins, Matthew J
• Format: Journal Article
• Language: English
• Published: Cambridge eLife Sciences Publications Ltd 23.08.2022; eLife Sciences Publications, Ltd
• Subjects: Amino acids; ATP/ADP; Beta cells; Calcium signalling; Cell activation; Cell Biology; Clonal deletion; Depolarization; Glucose; Glucose metabolism; Glycolysis; Insulin; Insulin secretion; Isoforms; katp channels; Membrane potential; Metabolism; Mitochondria; Oscillations; PEP cycle; Phosphorylation; Plasma; Pyruvate kinase; Pyruvic acid; Secretion; Workloads; β-cell metabolism
• ISSN: 2050-084X; 2050-084X
• DOI: 10.7554/eLife.79422

Pyruvate kinase (PK) and the phosphoenolpyruvate (PEP) cycle play key roles in nutrient-stimulated K ATP channel closure and insulin secretion. To identify the PK isoforms involved, we generated mice lacking β-cell PKm1, PKm2, and mitochondrial PEP carboxykinase (PCK2) that generates mitochondrial PEP. Glucose metabolism was found to generate both glycolytic and mitochondrially derived PEP, which triggers K ATP closure through local PKm1 and PKm2 signaling at the plasma membrane. Amino acids, which generate mitochondrial PEP without producing glycolytic fructose 1,6-bisphosphate to allosterically activate PKm2, signal through PKm1 to raise ATP/ADP, close K ATP channels, and stimulate insulin secretion. Raising cytosolic ATP/ADP with amino acids is insufficient to close K ATP channels in the absence of PK activity or PCK2, indicating that K ATP channels are primarily regulated by PEP that provides ATP via plasma membrane-associated PK, rather than mitochondrially derived ATP. Following membrane depolarization, the PEP cycle is involved in an ‘off-switch’ that facilitates K ATP channel reopening and Ca 2+ extrusion, as shown by PK activation experiments and β-cell PCK2 deletion, which prolongs Ca 2+ oscillations and increases insulin secretion. In conclusion, the differential response of PKm1 and PKm2 to the glycolytic and mitochondrial sources of PEP influences the β-cell nutrient response, and controls the oscillatory cycle regulating insulin secretion.