AAV-mediated hepatic expression of SLC30A10 and the Thr95Ile variant attenuates manganese excess and other phenotypes in Slc30a10-deficient mice

• Published in: The Journal of biological chemistry Vol. 300; no. 3; p. 105732
• Main Authors: Prajapati, Milankumar, Quenneville, Chelsea B., Zhang, Jared Z., Chong, Grace S., Chiu, Lauren, Ma, Bangyi, Ward, Lucas D., Tu, Ho-Chou, Bartnikas, Thomas B.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.03.2024; American Society for Biochemistry and Molecular Biology
• Subjects: AAV; Amino Acid Substitution; Animals; Body Weight; Brain - metabolism; Cation Transport Proteins - deficiency; Cation Transport Proteins - genetics; Cation Transport Proteins - metabolism; Cell Line; Dependovirus - genetics; Erythrocytes; genetic disease; Genome-Wide Association Study; Hepatocytes - metabolism; liver; Liver - cytology; Liver - metabolism; Liver Diseases - genetics; Liver Diseases - metabolism; manganese; Manganese - metabolism; Manganese Poisoning - metabolism; metal; Mice; Mutation; Phenotype; Promoter Regions, Genetic; SLC30A10; T95I; Thyroxine-Binding Globulin - genetics; toxicity; toxicity; Mn; AST; liver; EPO; DEG; metal; ALT; SLC30A10; manganese; AAV; TBG; genetic disease; T95I
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/j.jbc.2024.105732

The manganese (Mn) export protein SLC30A10 is essential for Mn excretion via the liver and intestines. Patients with SLC30A10 deficiency develop Mn excess, dystonia, liver disease, and polycythemia. Recent genome-wide association studies revealed a link between the SLC30A10 variant T95I and markers of liver disease. The in vivo relevance of this variant has yet to be investigated. Using in vitro and in vivo models, we explore the impact of the T95I variant on SLC30A10 function. While SLC30A10 I95 expressed at lower levels than T95 in transfected cell lines, both T95 and I95 variants protected cells similarly from Mn-induced toxicity. Adeno-associated virus 8-mediated expression of T95 or I95 SLC30A10 using the liver-specific thyroxine binding globulin promoter normalized liver Mn levels in mice with hepatocyte Slc30a10 deficiency. Furthermore, Adeno-associated virus–mediated expression of T95 or I95 SLC30A10 normalized red blood cell parameters and body weights and attenuated Mn levels and differential gene expression in livers and brains of mice with whole body Slc30a10 deficiency. While our in vivo data do not indicate that the T95I variant significantly compromises SLC30A10 function, it does reinforce the notion that the liver is a key site of SLC30A10 function. It also supports the idea that restoration of hepatic SLC30A10 expression is sufficient to attenuate phenotypes in SLC30A10 deficiency.