Genetic characterization of FSH beta-subunit gene and its association with buffalo fertility

Objective:To study genetic variation in buffalo follicle stimulating hormone beta-subunit (FSHB) gene and its association with fertility.Methods: In this experimental study, blood samples were collected by standard methods using EDTA anticoagulant and transrectal ultrasound examination was conducted...

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Bibliographic Details
Published inAsian Pacific Journal of Reproduction Vol. 6; no. 5; pp. 193 - 196
Main Authors Sosa, Ahmed, Mahmoud, Karima Gh, Kandiel, Mohamed, Eldebaky, Hazem, Nawito, Mahmoud, El-Roos, Mahmoud Abou
Format Journal Article
LanguageEnglish
Published Medknow Publications and Media Pvt. Ltd 01.09.2017
Department of Animal Reproduction & A.I, National Research Centre, Dokki, Tahrir Street, 12622 Giza, Egypt%Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Kaliobia, Egypt
Wolters Kluwer Medknow Publications
Subjects
Analysis
Anticoagulants
Beef cattle
Buffalo
DNA
DNA sequencing
Follicle-stimulating hormone
FSH beta-subunit gene
Genes
Genetic aspects
Genetic polymorphisms
Glycoproteins
Gonadotropins
Hormones
Nucleotide sequences
Single nucleotide polymorphisms
SSCP analysis
SSCP analysis
Nucleotide sequences
FSH beta-subunit gene
Buffalo
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Summary:Objective:To study genetic variation in buffalo follicle stimulating hormone beta-subunit (FSHB) gene and its association with fertility.Methods: In this experimental study, blood samples were collected by standard methods using EDTA anticoagulant and transrectal ultrasound examination was conducted on fertile (n=74) and infertile buffaloes with a history of anestrum (n=30) or repeat breeding (n=12). The genomic DNA was extracted for PCR followed by single strand conformation polymorphism analysis. DNA sequencing was performed for the determination of single nucleotide polymorphism ofFSHB gene.Results:The study results showed that there was genetic polymorphism with two different single strand conformation polymorphism patterns, AA and AB. The former pattern was associated with fertility in Egyptian buffaloes. Pair wise alignment of the two patterns sequences revealed that FSHB pattern II (AB) has C nucleotide insertion as SNP at the site of 208 bp of sequenced fragment.Conclusions:FSHB is polymorphic in the infertile Egyptian buffaloes, suggesting its practicability as a candidate marker for female fertility.
ISSN:2305-0500
2305-0519
DOI:10.4103/2305-0500.215928