Metabolite profiling and transcriptome analyses reveal defense regulatory network against pink tea mite invasion in tea plant

• Published in: BMC genomics Vol. 25; no. 1; pp. 989 - 17
• Main Authors: Chen, Limin, Shu, Zaifa, Zhou, Dayun, Zhou, Huijuan, Wang, Jinchao, Feng, Yaqi, Zheng, Shenghong, He, Weizhong
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 22.10.2024; BioMed Central; BMC
• Subjects: Analysis; Animals; Camellia sinensis - genetics; Camellia sinensis - metabolism; Camellia sinensis - parasitology; Catechin; Comparative analysis; Differential genes; Disease Resistance - genetics; Diseases and pests; Environmental aspects; Flavonoids; Gene Expression Profiling; Gene Expression Regulation, Plant; Gene Regulatory Networks; Genes; Genetic aspects; Influence; Isoflavones; Metabolites; Metabolome; Metabolomics; Mites; Oxidases; Phenylalanine; Pink tea mite; Plant Diseases - genetics; Plant Diseases - parasitology; Plant genetics; Plant metabolites; Proteins; Resistance; Tea; Tea (Plant); Tea production; Transcriptome; China; Resistance; Flavonoids; Pink tea mite; Tea production; Differential genes
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-024-10877-z

The tea plant Camellia sinensis (L.) O. Kuntze is a perennial crop, invaded by diversity of insect pest species, and pink tea mite is one of the most devastating pests for sustainable tea production. However, molecular mechanism of defense responses against pink tea mites in tea is still unknown. In this study, metabolomics and transcriptome profiles of susceptible and resistant tea varieties were compared before and after pink tea mite infestation. Metabolomics analysis revealed that abundance levels of polyphenol-related compounds changed significantly before and after infestation. At the transcript level, nearly 8 GB of clean reads were obtained from each sequenced library, and a comparison of infested plants of resistant and susceptible tea varieties revealed 9402 genes with significant differential expression. An array of genes enriched in plant pathogen interaction and biosynthetic pathways of phenylpropanoids showed significant differential regulation in response to pink tea mite invasion. In particular, the functional network linkage of disease resistant proteins, phenylalanine ammonia lyase, flavanone -3-hydroxylase, hydroxycinnamoyl-CoA shikimate transferase, brassinosteroid-6-oxidase 1, and gibberellin 2 beta-dioxygenase induced dynamic defense signals to suppress prolonged pink tea mite attacks. Further integrated analyses identified a complex network of transcripts and metabolites interlinked with precursors of various flavonoids that are likely modulate resistance against to pink tea mite. Our results characterized the profiles of insect induced metabolic and transcript reprogramming and identified a defense regulatory network that can potentially be used to fend off pink tea mites damage.