Association of long noncoding RNA and c-JUN expression in hepatocellular carcinoma

• Published in: Expert review of gastroenterology & hepatology Vol. 10; no. 7; p. 869
• Main Authors: El-Tawdi, Ahmed H F, Matboli, Marwa, El-Nakeep, Sarah, Azazy, Ahmed E M, Abdel-Rahman, Omar
• Format: Journal Article
• Language: English
• Published: England 02.07.2016
• Subjects: Area Under Curve; Biomarkers, Tumor - blood; Biomarkers, Tumor - genetics; Carcinoma, Hepatocellular - blood; Carcinoma, Hepatocellular - genetics; Carcinoma, Hepatocellular - mortality; Carcinoma, Hepatocellular - pathology; Case-Control Studies; Disease Progression; Disease-Free Survival; Female; Gene Expression Regulation, Neoplastic; Humans; Kaplan-Meier Estimate; Liver Neoplasms - blood; Liver Neoplasms - genetics; Liver Neoplasms - mortality; Liver Neoplasms - pathology; Male; Middle Aged; Neoplasm Recurrence, Local; Predictive Value of Tests; Proportional Hazards Models; Proto-Oncogene Proteins c-jun - blood; Proto-Oncogene Proteins c-jun - genetics; Real-Time Polymerase Chain Reaction; Risk Factors; RNA, Long Noncoding - blood; RNA, Long Noncoding - genetics; RNA, Messenger - blood; RNA, Messenger - genetics; ROC Curve; Time Factors; lncRNA-UCA1; C-JUN; prognosis; biomarkers; hepatocellular carcinoma; bioinformatics
• ISSN: 1747-4132
• DOI: 10.1080/17474124.2016.1193003

Long noncoding RNAs(lncRNAs) have emerged as key elements in modulating gene expression in different biological contexts. We used quantitative real-time PCR (Qpcr) to evaluate the expression of lncRNA-UCA1 and C-JUN in serum of 70 patients with hepatocellular carcinoma (HCC), 32 patients chronic hepatitis C (CHC) and 38 healthy subjects and their correlation with different clinicopathological factors. The expression of lncRNA-UCA1 and C-JUN was positive in 91.4%HCC patients with strong discriminating power between HCC and healthy subjects and CHC patients as well. The median follow up period was 29 months. The survival analysis showed that both lncRNA-UCA1 and C-JUN were independent prognostic factors. Of note, we identified C-JUN expression changes consistent with the lncRNA-UCA1 target regulation. This information sheds light on the possible role of lncRNA-UCA1 and C-JUN mRNA as promising diagnostic and prognostic markers as well as potential therapeutic targets in HCC.