Determination of Matrix Metalloproteinase 2 in Biological Samples Using a 3D Stochastic Microsensor Based on Graphene Oxide/AuNanoparticles/(Z)-N-(pyridin-4-yl-methyl) Octadec-9-enamide

• Published in: International journal of molecular sciences Vol. 25; no. 12; p. 6720
• Main Authors: Cioates Negut, Catalina, Ilie-Mihai, Ruxandra-Maria, Stefan-van Staden, Raluca-Ioana
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 18.06.2024; MDPI
• Subjects: biological samples; Biomarkers; Biosensing Techniques - methods; Cancer; Carbon; Gastric cancer; Gold - chemistry; Graphene; Graphite - chemistry; Growth factors; Humans; Investigations; Matrix Metalloproteinase 2 - blood; Matrix Metalloproteinase 2 - metabolism; Matrix Metalloproteinase 2 - urine; Metal Nanoparticles - chemistry; MMP-2; Morphology; Motility; Nanoparticles; Quantitative analysis; Sensors; Stomach Neoplasms - blood; Stomach Neoplasms - diagnosis; three-dimensional stochastic microsensor; Tumors; three-dimensional stochastic microsensor; MMP-2; gastric cancer; biological samples
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25126720

The levels of the MMPs in the biological samples of confirmed patients with gastric cancer are significantly elevated compared to those found in healthy people. Therefore, a novel 3D stochastic microsensor based on graphene oxide, modified with gold nanoparticles and (Z)-N-(pyridin-4-yl-methyl) octadec-9-enamide (namely N2-AuNP/GO), was designed for the determination of MMP-2 in biological samples, and validated for the screening tests of biological samples in order to be used for the early diagnosis of gastric cancer. The proposed sensor presents a low limit of quantification (1.00 × 10 g mL ), high sensitivity (1.84 × 10 s g mL), and a wide working concentration range (1.00 × 10 -1.00 × 10 g mL ). Recovery values higher than 99.15% were recorded for the assay of MMP-2 in whole blood, gastric tissue tumors, saliva, and urine samples.