Chromatin-modifying elements for recombinant protein production in mammalian cell systems

• Published in: Critical reviews in biotechnology Vol. 40; no. 7; pp. 1035 - 1043
• Main Authors: Guo, Xiao, Wang, Chong, Wang, Tian-Yun
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 02.10.2020
• Subjects: Animals; Cell Line; CHO Cells; Chromatin - genetics; chromatin-modifying element; Cricetinae; Cricetulus; expression vector; Genetic Vectors - genetics; HEK293 Cells; Humans; insulators; mammalian cell; matrix attachment region; Protein Engineering - methods; Recombinant protein; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Regulatory Sequences, Nucleic Acid - genetics; stabilizing anti-repressor elements; Ubiquitous chromatin open elements; mammalian cell; matrix attachment region; stabilizing anti-repressor elements; insulators; Ubiquitous chromatin open elements; chromatin-modifying element; Recombinant protein; expression vector
• ISSN: 0738-8551; 1549-7801
• DOI: 10.1080/07388551.2020.1805401

Mammalian cells are the preferred choice system for the production of complex molecules, such as recombinant therapeutic proteins. Although the technology for increasing the yield of proteins has improved rapidly, the process of selecting, identifying as well as maintaining high-yield cell clones is still troublesome, time-consuming and usually uncertain. Optimization of expression vectors is one of the most effective methods for enhancing protein expression levels. Several commonly used chromatin-modifying elements, including the matrix attachment region, ubiquitous chromatin opening elements, insulators, stabilizing anti-repressor elements can be used to increase the expression level and stability of recombinant proteins. In this review, these chromatin-modifying elements used for the expression vector optimization in mammalian cells are summarized, and future strategies for the utilization of expression cassettes are also discussed.