The fatty acid and monosaccharide compositions of three neutral and three phosphorylated glycolipids isolated from Leishmania donovani promastigotes grown in a chemically defined medium

Several lipids and macromolecular lipoconjugates of Leishmania spp. have now been well characterized; however, the glycolipids of L. donovani have not been thoroughly examined. In the present study, 3 neutral and 3 phosphorylated glycolipids were detected in promastigote forms of the organism grown...

Full description

Saved in:
Bibliographic Details
Published inThe Journal of parasitology Vol. 85; no. 5; p. 771
Main Authors Wyder, M A, Sul, D, Kaneshiro, E S
Format Journal Article
LanguageEnglish
Published United States 01.10.1999
Subjects
Animals
Chromatography, Thin Layer
Culture Media
Fatty Acids - analysis
Gas Chromatography-Mass Spectrometry
Glyceryl Ethers - analysis
Glyceryl Ethers - metabolism
Glycolipids - chemistry
Glycolipids - isolation & purification
Glycolipids - metabolism
Hydrolysis
Leishmania donovani - chemistry
Leishmania donovani - growth & development
Magnetic Resonance Spectroscopy
Monosaccharides - analysis
Phosphorylation
Online AccessGet more information

Cover

More Information
Summary:Several lipids and macromolecular lipoconjugates of Leishmania spp. have now been well characterized; however, the glycolipids of L. donovani have not been thoroughly examined. In the present study, 3 neutral and 3 phosphorylated glycolipids were detected in promastigote forms of the organism grown in a chemically defined medium. The fatty acid and sugar compositions of these glycolipids, isolated and purified by adsorption column chromatography and thin-layer chromatographic procedures, were identified and quantified by gas-liquid chromatography and mass spectrometry. Myristate (14:0), palmitate (16:0), palmitoleate (16:1), stearate (18:0), oleate (18:1), and linoleate (18:2) were the major fatty acids in all 6 glycolipids. Arabinose, mannose, glucose, and galactose were detected in the glycolipids. The biochemical nature of these lipids suggested that the major components in the isolated preparations of the 6 glycolipids are diacylglycerophospholipids, distinct from the major precursors of macromolecular lipoconjugates such as the lipid anchors of cell surface antigens that have been reported. These appear to be terminal products of lipid biosynthesis in this parasite.
ISSN:0022-3395
DOI:10.2307/3285810